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圖爾斯rna seq 在 [討論] 推薦的RNA seq廠商- 看板Biotech - 批踢踢實業坊 的推薦與評價


作者michaelhsien (MichaelHsien)
看板Biotech
標題[討論] 推薦的RNA seq廠商
時間Mon Jul 11 10:07:26 2016

哈囉大家好
我的研究想要做RNA seq
詢問了幾家廠商，發現有不少人在做
像是 基龍米克斯、源資、圖爾斯…等等（非廣告）
但是不知道這幾家廠商定序品質及事後分析服務是否完善
(我們不是很擅長生物資訊方面，所以希望事後分析要夠好...)

請問板上的各位
是否有推薦的廠商
或是很雷的廠商…QQ

--
※ 發信站: 批踢踢實業坊(ptt.cc), 來自: 101.10.22.63
※ 文章網址: https://www.ptt.cc/bbs/Biotech/M.1468202849.A.4CD.html
推 jabari: 都很雷啊... 簡單作法 叫每間都報 討論你要怎麼作 07/11 13:18
→ jabari: 如果你們lab本身找不到生資分析人員 我建議先找別的lab合 07/11 13:19
→ jabari: 作 然後再請他們推薦要找哪間跑RNA seq 07/11 13:19
推 silverberry: 淚推樓上! 07/11 16:05
推 chong: 很多都是外包，收件後送到北京華大定序分析 07/11 17:23

其實我比較在意定序後的分析...
不知道jabari和silverberry有什麼辛酸血淚史嗎...?
※ 編輯: michaelhsien (61.230.145.1), 07/11/2016 20:39:28
推 michealking: 值拿回來 丟IPA 07/11 21:03
推 lelojack: 廠商的好處是報告弄的漂漂亮亮的 很好理解，但沒法跟你 07/11 21:14
→ lelojack: 分析到最後， 學術合作可以跟你磨到後面，但報告的UI做 07/11 21:14
→ lelojack: 不好 07/11 21:14
推 abcam: 台灣的廠商都外送到大陸了～根本沒有在台灣做啊 07/11 21:17
→ abcam: 面對中國的產品與服務，我們就要面對他囉～(老闆說的)datab 07/11 21:18
→ abcam: ase 真的很龐大 07/11 21:18
推 huuban: 看實驗目的吧..廠商能出圖表但不見得是你們想要的東西啊 07/11 22:07
推 lingon: 不會做生資別花錢做RNASeq, 廠商的分析也不要太信 07/11 22:22
推 jabari: 其實我想說RNAseq退流行了 現在有protein scan的... 07/11 22:27
→ blence: 應該先問,你想得到怎樣的data,以及只有RNAseq能解決問題? 07/11 23:00
→ blence: 現在已經有許多OA data,廠商能給的也不脫離這些內容,如果 07/11 23:03
→ blence: 你真的想送樣,至少先知道後續會遇到的困難 07/11 23:06
推 Alstonia: 先決定你要用的平台跟分析的方向或者有paper可以當範本 07/11 23:37
→ Alstonia: 舉例，再看看你預算的上限是多少，拿著這些去跟廠商討 07/11 23:38
→ Alstonia: 論。如果完全沒經驗的話建議跟樓上先進說的一樣找人合作 07/11 23:38
→ Alstonia: ，data產出後面的工作還很多，很難錢丟了就解決， 07/11 23:38
推 Alstonia: 講具體一點就是可以先想想你的論文或報告裡希望能畫出 07/11 23:48
→ Alstonia: 怎樣的圖跟比較表，把這些需求同時跟廠商討論這樣會比 07/11 23:48
→ Alstonia: 較清楚。 07/11 23:48
推 Alstonia: 然後在台灣自己有機器自己跑的也還不少家，被對岸價格 07/11 23:52
→ Alstonia: 壓著打就是了。 07/11 23:52
推 BGG: 要不要先做QPCR array看看mRNA表現量差異 07/11 23:58
推 jabari: 先問清楚 這個計畫一定得跑RNAseq嗎? 還是他已經寫死 你一 07/12 00:02
→ jabari: 定得上... 或者是你們有候選基因了 只是跑一下到時候其中 07/12 00:03
→ jabari: 報告好過關 因為這個坑很大啊...沒有生資背景的拿到會被騙 07/12 00:03
→ jabari: 很大 07/12 00:03
推 lingon: 建議你跟你老闆先看這:https://tinyurl.com/jrqky4z 07/12 00:44
→ lingon: 你跟老闆從第28張開始看,看不懂的話做RNASeq很容易被騙 07/12 00:46
→ lingon: 至於protein scan目前還只是炫而已...而且用途不同 07/12 00:48
推 lingon: 雖然能夠很直接量蛋白量,但能量到的蛋白還是有限,準度也.. 07/12 00:54
推 jabari: 我認識一位受害者...欸 老闆你來啦?? 07/12 03:31
→ sunorange: 廠商都很雷，給的東西完全不能用 07/12 17:12
→ sunorange: 我們後來都自行分析了，如果沒有分析能力 07/12 17:13
→ sunorange: 建議不要做，浪費錢而已 07/12 17:13






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圖爾斯rna seq 在 GEP Tools | Using the RNA-Seq Tracks - YouTube 的推薦與評價

影片讀取中

A video describing RNA - Seq by Virtual GEP TA Christina Ray Santos. We start off with a guided navigation to the GEP UCSC Genome Browser, ... ... <看更多>

圖爾斯rna seq 在 Functional Analysis for RNA-seq | Introduction to DGE 的推薦與評價

Determine how functions are attributed to genes using Gene Ontology terms; Understand the theory of how functional enrichment tools yield statistically enriched ... ... <看更多>

圖爾斯rna seq 在 PF2-pasteur-fr/SARTools: Statistical Analysis of RNA-Seq ... 的推薦與評價

Statistical Analysis of RNA-Seq Tools. Contribute to PF2-pasteur-fr/SARTools development by creating an account on GitHub. ... <看更多>

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RNA sequencing technology (RNA-Seq) has become the most ... tools to precisely predict differentially expressed genes (DEGs) from RNA-Seq data.

#67. kallisto | bustools

Generate a cell x gene or cell x transcript equivalence class count matrix · Perform RNA velocity and single-nuclei RNA-seq analsis · Quantify data from numerous ...

#68. Introduction to RNA-seq and functional interpretation

This course will provide an introduction to the technology, data analysis, tools and resources used in RNA sequencing and transcriptomics.

#69. FlyBase:RNA-Seq Overview

The RNA-Seq query tools are restricted to the modENCODE datasets. For each modENCODE RNA-Seq sample, gene expression level was calculated as ...

#70. Rna Seq Analysis Tools Full PDF

In today digital age, eBooks have become a staple for both leisure and learning. The convenience of accessing Rna Seq Analysis Tools and various genres has ...

#71. Evaluation of Seven Different RNA-Seq Alignment Tools ...

Quantification of gene expression is crucial to connect genome sequences with phenotypic and physiological data. RNA-Sequencing (RNA-Seq) ...

#72. Efficient Tools for Quantifying and Simulating Transcript- ...

Results & Resources. First, the Patro lab built a method for uncertainty quantification for droplet-based single-cell RNA-seq quantification—implemented as the ...

#73. [討論] 推薦的RNA seq廠商- 看板Biotech - 批踢踢實業坊

哈囉大家好我的研究想要做RNA seq 詢問了幾家廠商，發現有不少人在做像是基龍米克斯、源資、圖爾斯…等等（非廣告） 但是不知道這幾家廠商定序品質及 ...

#74. Python Tools For Single Cell RNA-seq Analysis

RNA -seq is a revolutionary and powerful transcriptomics technique that facilitates the study of gene expression by taking advantage of the ...

#75. Functional Analysis for RNA-seq | Introduction to DGE

Determine how functions are attributed to genes using Gene Ontology terms; Understand the theory of how functional enrichment tools yield statistically enriched ...

#76. Tools for genetics and genomics: Gene expression profiling

Microarrays (oligonucleotide arrays) · Transcriptome sequencing (RNA-seq) · Single-cell RNA sequencing · In situ and spatial transcriptomics.

#77. Evaluation of tools for differential gene expression analysis ...

ABSTRACT. An RNA‐seq experiment with 48 biological replicates in each of 2 conditions was performed to determine the number of biological replicates ...

#78. RNA-seq analysis using long and short reads from ...

The host and pathogen reference genomes are downloaded and combined into one synthetic reference using QIAGEN CLC Genomics Workbench tools. The short and long ...

#79. PF2-pasteur-fr/SARTools: Statistical Analysis of RNA-Seq ...

Statistical Analysis of RNA-Seq Tools. Contribute to PF2-pasteur-fr/SARTools development by creating an account on GitHub.

#80. RNA-seq Analysis

GenePattern offers a set of tools to support a wide variety of RNA-seq analyses, including short-read mapping, identification of splice junctions, transcript ...

#81. Over 1000 tools reveal trends in the single-cell RNA-seq ...

Recent years have seen a revolution in single-cell RNA-sequencing (scRNA-seq) technologies, datasets, and analysis methods. Since 2016, the scRNA-tools database.

#82. RNA-Seq Data Analysis in Galaxy

A complete RNA-Seq analysis involves the use of several different tools, with substantial software and computational requirements.

#83. Bioinformatics Tools for RNA-seq Gene and Isoform ...

Recently, a number of tools have been developed for RNA-seq isoform quantification. We review those methods and their main features to serve as guidance for ...

#84. Tools for Single Cell Genomics • Seurat

Seurat is an R package designed for QC, analysis, and exploration of single-cell RNA-seq data. Seurat aims to enable users to identify and interpret sources ...

#85. Comparative analysis of differential gene expression tools for ...

RNA sequencing (RNA-seq) has become a standard procedure to investigate transcriptional changes between conditions.

#86. 9 factors to consider when choosing RNA-seq analysis ...

These tools suit scientists with any level of data analysis expertise and can accelerate research and drug discovery workflows. This article ...

#87. Application of deconvolution tools on RNA-Seq data

tools on RNA-Seq data. Emeline, Hélène, Natalia. Sys Bio group meeting. 03/06/2021. Page 2. Context and data. Sylvain Baize unit, Biology of Viral Emerging ...

#88. About Single-Cell RNA-Sequencing | Collections

Specialised tools are required for nearly all the stages of the scRNA-seq analysis workflow. While there is already a plethora of existing tools, new tools ...

#89. UMI-tools: modeling sequencing errors in Unique ...

Reproducibility between iCLIP replicates and single-cell RNA-seq clustering are both improved using our proposed network-based method, ...

#90. Galaxy

Interactive tools. Operate on Genomic Intervals. Fetch Sequences/Alignments. Genomics Analysis. Assembly. Annotation. Mapping. Variant Calling. ChIP-seq. RNA- ...

#91. Comparing HISAT and STAR-based pipelines for RNA-Seq ...

In literature, there are several tools implemented by practitioners and researchers for the alignment step. However, two tools are the de-facto-standard ...

#92. Packages found under RNASeq

Modeling RNA synthesis, processing and degradation with RNA-seq data, 1205. isomiRs ... Tools for finding Total RNA Expression Genes in single nucleus RNA-seq ...

#93. Best RNA-Seq aligner: A comparison of mapping tools

What software tools should be used for the alignment of RNA sequencing reads from NGS.

#94. NGS Quality Control in RNA Sequencing- Some Free Tools

RNA sequencing ('RNA-seq') has become one of the most widely used applications for Next-Generation Sequencing. RNA-seq can provide gene ...

#95. Robust discovery and quantification of transcript isoforms ...

Long-read RNA sequencing (RNA-seq) holds great potential for ... tools for long-read RNA-seq transcript quantification (Fig. 2D and ...

#96. Wellesley Computer Scientists Develop DNA/RNA ...

Wellesley Computer Scientists Develop DNA/RNA Sequencing Tools. Tools Developed in Wellesley Computer Science Labs Aid Biologists, Medical ...

#97. RNAseq定序服務(mRNA Sequencing)

... (mRNA Sequencing). RNAseq定序服務. 特色說明. mRNA 定序目前廣泛應用於細胞轉錄體和基因表達變化的研究，結合生物資訊分析，得以比較不同實驗設計條件下，樣本間轉錄 ...

#98. RNA-seq

Transcripts provides a set of tools for analyzing RNA-seq data. Tools provided in this package can be easily wired together to form a workflow for routine RNA- ...

#99. Specialized RNA-Seq Analysis Tools

Specialized RNA-Seq Analysis Tools. Transcriptome/RNA-seq, Expression and Alternative Splicing Analysis. Next Generation RNA sequencing is a powerful tool for ...